文献类型：期刊文献

英文题名：Enhanced immune response to DNA-based HPV16L1 vaccination by costimulatory molecule B7-2

作者：Zheng, J; Si, LS; Song, JM; Sun, XL; Yu, J; Wang, YL

第一作者：Zheng, J

通讯作者：Wang, YL[1]

机构：[1]Xi An Jiao Tong Univ, Coll Life Sci & Technol, Inst Canc Res, Xian 710061, Peoples R China;[2]Univ London Imperial Coll Sci Technol & Med, Fac Med, Dept Paediat, Mol Infect Dis Grp, London W2 1PG, England

通讯机构：[1]corresponding author), Xi An Jiao Tong Univ, Coll Life Sci & Technol, Inst Canc Res, Xian 710061, Peoples R China.

年份：2003

卷号：59

期号：1

起止页码：61-65

外文期刊名：ANTIVIRAL RESEARCH

收录：;Scopus(收录号：2-s2.0-0038798620);WOS:【SCI-EXPANDED(收录号:WOS:000184106400008)】；

语种：英文

外文关键词：human papillomavirus; DNA-vaccination; costimulatory molecule

摘要：We have investigated whether co-injection of DNA encoding the costimulatory molecule B7-2 augments immune response to the major capsid protein L1 of the high-risk human papillomavirus type 16 (HPV16L1). While immunoglobulin G (IgG) specific to HPV16LI was detected in sera from mice injected intramuscularly with pcDNA-L1 that encodes HPV16L1, a significantly increased level of IgG was found in sera from mice immunised with pcDNA-L1 in conjunction with pLXHDmB7-2 DNA. Level,; of IgG in the anti-sera were correlated with the inhibitory activity of the murine erythrocyte hemagglutination caused by the virus-like particles (VLP) and the binding of VLP to HeLa. cells. Moreover, splenic cells isolated from mice co-injected with pLXHDmB7-2 had stronger proliferation and more IFN-gamma producing T cells (CD4(+) and CD8(+)) when stimulated with HPV16 VLP compared with cells from mice that had received pcDNA-L1 alone and mice of the control groups. Furthermore, in footpad swelling test, mice co-immunised with pLXHDmB7-2 had greater skin thickness over those immunised with pcDNA-L1 alone or control mice. We conclude that co-injection of DNA encoding B7-2 can enhance both humoral and cellular immune responses elicited by DNA-based vaccination against HPV16 infection in mice. (C) 2003 Elsevier Science B.V. All rights reserved.