文献类型：期刊文献

英文题名：A systematic investigation of key factors of nucleic acid precipitation toward optimized DNA/RNA isolation

作者：Li, Yalin[1];Chen, Suxiang[2,3];Liu, Nan[4];Ma, Lixia[5];Wang, Tao[6];Veedu, Rakesh N.[2,3];Li, Tao[1];Zhang, Fengqiu[1];Zhou, Huiyue[6];Cheng, Xiang[4];Jing, Xumiao[1]

第一作者：Li, Yalin

通讯作者：Zhang, FQ[1];Wang, T[2]

机构：[1]Zhengzhou Univ, Sch Phys, Henan Key Lab Ion Beam Bioengn, Zhengzhou 450000, Peoples R China;[2]Murdoch Univ, Ctr Mol Med & Innovat Therapeut, Perth, WA 6150, Australia;[3]Perron Inst Neurol & Translat Sci, Perth, WA 6150, Australia;[4]Guangzhou Med Univ, Sch Publ Hlth, Guangzhou 510000, Peoples R China;[5]Henan Univ Econ & Law, Sch Stat, Zhengzhou 450000, Peoples R China;[6]Sch Nursing, Zhengzhou 450000, Peoples R China

第一机构：Zhengzhou Univ, Sch Phys, Henan Key Lab Ion Beam Bioengn, Zhengzhou 450000, Peoples R China

通讯机构：[1]corresponding author), Zhengzhou Univ, Sch Phys, Henan Key Lab Ion Beam Bioengn, Zhengzhou 450000, Peoples R China;[2]corresponding author), Sch Nursing, Zhengzhou 450000, Peoples R China.

年份：2020

卷号：68

期号：4

起止页码：191-199

外文期刊名：BIOTECHNIQUES

收录：;Scopus(收录号：2-s2.0-85083546654);WOS:【SCI-EXPANDED(收录号:WOS:000527531400005)】；

基金：This work was supported by the Natural Science Foundation of China (grant nos. 11704343, 11605159 and 81773175), the China Postdoctoral Science Foundation (grant no. 2018M630839), the College Key Research Project in Henan province (grant no. 18A180032) and the Science and Technology Research Project in Henan province (grant nos 162102210155, 192102310299). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

语种：英文

外文关键词：ethanol; isopropanol; linear polyacrylamide; nucleic acid precipitation

摘要：Nucleic acid precipitation is important for virtually all molecular biology investigations. However, despite its crucial role, a systematic study of the influence factors of nucleic acid precipitation has not been reported. In the present work, via rational experimental design, key factors of nucleic acid precipitation, including the type of nucleic acid, temperature and time of incubation, speed and time of centrifugation, volume ratio of ethanol/isopropanol to nucleic acid solution, type of cation-containing salt solution and type of coprecipitator, were comprehensively evaluated in an attempt to maximize the efficiency of nucleic acid precipitation. Our results indicate that the optimal conditions of each influence factor of nucleic acid precipitation may vary in accordance with the chemistry, structure and length of nucleic acids. METHOD SUMMARY To maximize the efficiency of nucleic acid precipitation, the key factors, including incubation and centrifugation conditions, and the volume ratio of polar solvents, cation-containing salts and coprecipitators, were systematically evaluated. To gain more practical information, four types of commonly used nucleic acids were tested. The results indicate that the optimal conditions vary greatly in accordance with the type of nucleic acids under study.