文献类型：期刊文献

英文题名：Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics

作者：Yang, Dongbin[1,2];Zhang, Weihong[3];Zhang, Huanyun[1,2];Zhang, Fengqiu[4];Chen, Lanmei[5];Ma, Lixia[6];Larcher, Leon M.[7];Chen, Suxiang[7];Liu, Nan[8];Zhao, Qingxia[9];Tran, Phuong H. L.[10,11];Chen, Changying[12];Veedu, Rakesh N.[7,13];Wang, Tao[3,7,13]

第一作者：Yang, Dongbin

通讯作者：Wang, T[1];Veedu, RN[2];Wang, T[2];Chen, CY[3];Veedu, RN[4];Wang, T[4]

机构：[1]Hebi Peoples Hosp, Dept Neurosurg, Hebi 458030, Peoples R China;[2]Hebi Neuroanat Lab, Hebi 458030, Peoples R China;[3]Zhengzhou Univ, Sch Nursing, Zhengzhou 450001, Peoples R China;[4]Zhengzhou Univ, Henan Key Lab Ion Beam Bioengn, Zhengzhou 450000, Peoples R China;[5]Guangdong Med Univ, Sch Pharm, Guangdong Key Lab Res & Dev Nat Drugs, Zhanjiang 524023, Peoples R China;[6]Henan Univ Econ & Law, Sch Stat, Zhengzhou 450046, Peoples R China;[7]Murdoch Univ, Ctr Mol Med & Innovat Therapeut, Perth, WA 6150, Australia;[8]Southern Med Univ, Nanhai Hosp, Gen Practice Ctr, Foshan 528244, Peoples R China;[9]Wake Forest Univ, Bowman Gray Sch Med, Winston Salem, NC 27101 USA;[10]Deakin Univ, Sch Med, Geelong, Vic 3216, Australia;[11]Deakin Univ, Ctr Mol & Med Res, Geelong, Vic 3216, Australia;[12]Zheng Zhou Univ, Affiliated Hosp 1, Zhengzhou 450001, Peoples R China;[13]Perron Inst Neurol & Translat Sci, Perth, WA 6009, Australia

第一机构：Hebi Peoples Hosp, Dept Neurosurg, Hebi 458030, Peoples R China

通讯机构：[1]corresponding author), Zhengzhou Univ, Sch Nursing, Zhengzhou 450001, Peoples R China;[2]corresponding author), Murdoch Univ, Ctr Mol Med & Innovat Therapeut, Perth, WA 6150, Australia;[3]corresponding author), Zheng Zhou Univ, Affiliated Hosp 1, Zhengzhou 450001, Peoples R China;[4]corresponding author), Perron Inst Neurol & Translat Sci, Perth, WA 6009, Australia.

年份：2020

卷号：10

期号：8

起止页码：3684-3707

外文期刊名：THERANOSTICS

收录：;Scopus(收录号：2-s2.0-85080885251);WOS:【SCI-EXPANDED(收录号:WOS:000518768400021)】；

基金：D.Y, W.Z, H.Z, F.Z, C.C, T.W acknowledge the funding from the National Natural Science Foundation of China (No.81773175, No.11704343, and U1404814) and the China Postdoctoral Science Foundation (No. 2018M630839); T.W and R.N.V are supported by Murdoch University, and RNV also acknowledges the funding support from the McCusker foundation through Perron Institute of Neurological and Translational Sciences. T.W is also supported by Murdoch University commercial research funding and Perron early and mid-career researcher internal grants scheme; T. P is the recipient of Australian Research Council's Discovery Early Career Researcher Award (project number DE160100900).

语种：英文

外文关键词：Exosome; microvesicle; extracellular vesicle; microfluidic; diagnosis; separation

摘要：Exosomes are small extracellular vesicles with diameters of 30-150 nm. In both physiological and pathological conditions, nearly all types of cells can release exosomes, which play important roles in cell communication and epigenetic regulation by transporting crucial protein and genetic materials such as miRNA, mRNA, and DNA. Consequently, exosome-based disease diagnosis and therapeutic methods have been intensively investigated. However, as in any natural science field, the in-depth investigation of exosomes relies heavily on technological advances. Historically, the two main technical hindrances that have restricted the basic and applied researches of exosomes include, first, how to simplify the extraction and improve the yield of exosomes and, second, how to effectively distinguish exosomes from other extracellular vesicles, especially functional microvesicles. Over the past few decades, although a standardized exosome isolation method has still not become available, a number of techniques have been established through exploration of the biochemical and physicochemical features of exosomes. In this work, by comprehensively analyzing the progresses in exosome separation strategies, we provide a panoramic view of current exosome isolation techniques, providing perspectives toward the development of novel approaches for high-efficient exosome isolation from various types of biological matrices. In addition, from the perspective of exosome-based diagnosis and therapeutics, we emphasize the issue of quantitative exosome and microvesicle separation.