文献类型：期刊文献

英文题名：Anti-tumor T cell response and protective immunity in mice that received sublethal irradiation and immune reconstitution

作者：Ma, J; Urba, WJ; Si, LS; Wang, YL; Fox, BA; Hu, HM

第一作者：Ma, J

通讯作者：Hu, HM[1]

机构：[1]Providence Portland Med Ctr, Earle A Chiles Res Inst, Robert W Franz Canc Res Ctr, Lab Canc Immunobiol, Portland, OR 97213 USA;[2]Providence Portland Med Ctr, Earle A Chiles Res Inst, Lab Mol & Tumor Immunol, Portland, OR USA;[3]Xi An Jiao Tong Univ, Sch Life Sci, Inst Immunopathol, Xian 710049, Peoples R China;[4]Oregon Hlth & Sci Univ, Dept Mol Microbiol & Immunol, Portland, OR 97201 USA;[5]Oregon Hlth & Sci Univ, Dept Environm & Biomol Syst, Portland, OR 97201 USA

通讯机构：[1]corresponding author), Providence Portland Med Ctr, Earle A Chiles Res Inst, Robert W Franz Canc Res Ctr, Lab Canc Immunobiol, 4805 NE Gilsan St, Portland, OR 97213 USA.

年份：2003

卷号：33

期号：8

起止页码：2123-2132

外文期刊名：EUROPEAN JOURNAL OF IMMUNOLOGY

收录：;Scopus(收录号：2-s2.0-0041975925);WOS:【SCI-EXPANDED(收录号:WOS:000184648700008)】；

语种：英文

外文关键词：T lymphocyte; melanoma vaccine; lymphopenia; reconstitution

摘要：To test whether homeostasis-driven T cell proliferation in reconstituted lymphodepleted hosts would improve the therapeutic efficacy of tumor vaccines, normal mice and reconstituted lymphopenic mice (RLM; C57BL/6 mice rendered lymphopenic with sublethal total-body irradiation and reconstituted with naive splenocytes) were used in the vaccination and challenge experiments with weakly immunogenic F10 melanoma cells. Only limited protection was observed in vaccinated normal mice (16.7%), whereas significantly greater protection was induced in vaccinated RLM (63.2%). Protective immunity in RLM depended on CD8 T cells. Following vaccination, a significant increase in the percentage of CD44(hi)CD62L(lo) T cells was detected in the tumor vaccine-draining lymph node (TVDLN) of vaccinated RLM compared to that of vaccinated normal mice. After in vitro stimulation, effector T cells generated from TVDLN of vaccinated FILM produced more IFN-gamma than T cells from vaccinated normal mice, and contained more melanoma-specific T cells, as assessed by ELISA and intracellular cytokine staining. This study suggests that vaccination of reconstituted lymphopenic hosts could elicit superior anti-tumor immunity compared to normal hosts, highlighting the potential clinical benefit of performing tumor vaccination during immune reconstitution.