文献类型：期刊文献

英文题名：Regulation through microRNAs in Response to Low-Energy N+ Ion Irradiation in Oryza sativa

作者：Li, Yalin[1];Wang, Weidong[1];Wang, Tao[2];Wouters, Merridee A.[3];Yin, Yue[1];Jiao, Zhen[1,4];Ma, Lixia[5];Zhang, Fengqiu[1]

第一作者：Li, Yalin

通讯作者：Jiao, Z[1];Zhang, FQ[1]

机构：[1]Zhengzhou Univ, Henan Key Lab Ion Beam Bioengn, 75 Daxue Rd, Zhengzhou 450000, Henan, Peoples R China;[2]Zhengzhou Univ, Sch Nursing, Zhengzhou 450000, Henan, Peoples R China;[3]La Trobe Univ, Sch Canc Med, Olivia Newton John Canc Res Inst, Bundoora, Vic 3086, Australia;[4]Zhengzhou Univ, Zhengzhou Res Base State Key Lab Cotton Biol, Zhengzhou 450000, Henan, Peoples R China;[5]Henan Univ Finance & Econ, Sch Stat, Zhengzhou 450000, Henan, Peoples R China

第一机构：Zhengzhou Univ, Henan Key Lab Ion Beam Bioengn, 75 Daxue Rd, Zhengzhou 450000, Henan, Peoples R China

通讯机构：[1]corresponding author), Zhengzhou Univ, Henan Key Lab Ion Beam Bioengn, 75 Daxue Rd, Zhengzhou 450000, Henan, Peoples R China.

年份：2019

卷号：191

期号：2

起止页码：189-200

外文期刊名：RADIATION RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000458110800006)】；

基金：This work was supported by the National Natural Science Fund of China (11704343 11605159 and 81773175), China Postdoctoral Science Foundation Grant (2018M630839), College Key Research Project in Henan province (18A180032), Science and Technology Research Project in Henan province (162102210155) and the Foundation for University Key Teachers of Henan Province.

语种：英文

摘要：MicroRNAs (miRNAs) are a non-coding regulatory RNAs that play significant roles in plant growth and development, especially in the stress response. Low-energy ion radiation, a type of environmental stress, can cause multiple biological effects. To understand the roles of miRNAs in response to low-energy N+ ion radiation in Oryza saliva, high-throughput sequencing of small RNAs was carried out to detect the expression of miRNAs in the shoots of the rice after 2 x 10(17) N+/cm(2) irradiation. The differentially expressed 28 known miRNAs were identified, 17 of these identified miRNAs were validated by real-time quantitative fluorescent PCR (q-PCR), including 9 up-regulated miRNAs (miR1320-3p, miR1320-5p, miR156b-3p, miR156c-5p, miR156c-3p/g-3p, miR1561-5p, miR398b and miR6250) and 8 down-regulated miRNAs (miR156a/e/i, miR156k, miR160f-5p, miR166j-5p, miR1846e and miR399d). In addition, 45 novel radiation-responsive miRNAs were predicted, and 8 of them were verified by q-PCR. The target genes of radiation-responsive miRNAs were predicted and gene function enrichment analysis was performed with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The expression of 9 targets of 4 known miRNA families (miR156, miR399, miR1320 and miR398) and 2 targets of 2 novel miRNAs were quantified by q-PCR, and a strong negative regulation relation between miRNAs and their targets were observed. Those targets including SQUAMOSA promoterbinding-like protein (SPL) genes, copper/zinc superoxide dismutase (Cu/Zn-SOD), copper chaperone for SOD (CCS1) and electron transporter/ heat-shock protein binding protein (HSP), which are involved in growth and defense against various stresses, especially associated with reactive oxygen species (ROS) scavenging. This work provides important information for understanding the ROS generation and elimination mechanisms closely related to miRNAs in rice seedlings after low-energy N+ radiation exposure. (C) 2019 by Radiation Research Society